histopathology of rainbow trout gills after exposure to copper

Copper uptake across rainbow trout gills: mechanisms of apical entry.

Several components of branchial copper uptake were identified in juvenile freshwater rainbow trout (Oncorhynchus mykiss) using (64)Cu. On the basis of competitive interactions between sodium and copper uptake, inhibition of copper uptake by a proton pump inhibitor (bafilomycin A1, 2 micromol l(-1)) and a Na(+) channel blocker (phenamil, 100 micromol l(-1)), it appears that a proportion of the b...

Primary Cultures of Epithelial Cells from Rainbow Trout Gills

A method for obtaining primary cultures of epithelial cells from rainbow trout gills is described. The yield of cells from approximately 1.5g wet mass of tissue was 2183106±123106 cells with a viability defined by eosin exclusion of 80±6%. Cells were seeded in culture dishes and grown in Leibowitz L-15 medium supplemented with 5 % foetal bovine serum. Attachment efficiency after 24h was 35±6%. ...

Cadmium inhibition of Ca2+ uptake in rainbow trout gills

Cadmium inhibition of Ca2+ uptake in rainbow trout gills.

The effects of cadmium (Cd2+) on calcium (Ca2+) transport in the gills of rainbow trout (Salmo gairdneri) were studied. The gill epithelium of freshwater fish represents a model for a Ca2+-transporting tight epithelium. Unidirectional Ca2+ fluxes in the gills were estimated in an isolated saline-perfused head preparation. Ca2+ influx was not affected when up to 10 microM Cd were added to the ve...

Waterborne vs. dietary copper uptake in rainbow trout and the effects of previous waterborne copper exposure.

Juvenile rainbow trout (Oncorhynchus mykiss) were exposed to waterborne Cu (22 microg/l) in moderately hard water for up to 28 days. Relative to control fish kept at background Cu levels (2 microg/l), Cu-preexposed fish displayed decreased uptake rates of waterborne Cu via the gills but not of dietary Cu via the gut during 48-h exposures to (64)Cu-radiolabeled water and diet, respectively. At n...

Copper metabolism and gut morphology in rainbow trout (Oncorhynchus mykiss) during chronic sublethal dietary copper exposure

Juvenile rainbow trottt (Oncorhynchus mykiss) werc exposed to I I (control), 300 (medium), and 1000 pg Cug-r (high) (as CuSOa.5H2O) in the diet for 28 days at a daily ration of 4%o wet body weight, with a background waterborne Cu concentration of3 pg.L-|. There was no effect ofdietary Cu on growth, condition factor, or food conversion efficiency. Whole-body Cu content increased continuously ove...